IMV Executive Assistant
tel. (612) 624-1926
fax. (612) 625-1108
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Minneapolis, MN 55455
Somia's interests are in the development and refinement of retrovirus vectors as tools for gene therapy and gene discovery.
Our ability to transfer genes into cells is at the heart of the concept of gene therapy (Somia and Verma, 2000). The retrovirus life cycle can be subverted so that the virus will ferry therapeutic genes into cells, as opposed to its normal pathogenic cargo. Although the methods already developed are sophisticated and allow high levels of gene transfer, one of the next steps in the development of vectors is to target infections to certain cell types. This will have great utility in organs such as the brain, which is composed of a complex mix of heterogeneous cells, as well as uses in systems where the cells are dispersed through the body - such as blood. Since the present vectors infect most cell types, they also include cells such as dendritic cells - the professional antigen presenting cells of the immune system. This could generate an immune response against the vector, the therapeutic gene product or both. In this regard, targeted vectors are important in the cell types that they don't infect. Presently the Somia lab is developing and refining new envelope proteins, which would mediate binding to the target cell and facilitate entry of the vector RNA in the cell (Somia et al., 1995, and Somia et. al., 2000).
More recently Somia has been interested in developing these high efficiency gene transfer tools towards gene discovery. To this end the lab has generated cDNA libraries in retroviral vectors enabling us to transfer entire complements of coding RNA from one cell type to another. If the lab can devise a suitable screen for the target cell, this technology enables us to discover function for the cDNA encoded by the retroviral vector. In initial screens we focused on programmed cell death, or apoptosis, caused by a molecule called Fas. Somia generated a cDNA library from a cell line that is resistant to Fas mediated cell death, transferred the library into a cell line that was sensitive to Fas mediated cell death, and cloned a novel gene for a protein that confers resistance to Fas (Somia et al., 1999). The Somia lab is currently dissecting this new gene and learning about its function and howit mediates the protection to apoptosis, a process that is important in development, the immune system, and in cancer. The lab wants to expand on this technology and have a number of different screens that will allow us to clone and identify the function of genes in diverse processes such as instem cells, in transcription and in viral infection - which connects us back to the interest in gene therapy.
'Wisc-e-sota', the 1st Joint UMN-UW Virology Training Grant Symposium will be held on Friday, Sepbember 20th, 2013 at the Uniiversity of Wisconsin-La Crosse, Cartwright Center. This is the inaugural collaborative symposium of the NIH T32-supported virology training programs at the University of Wisconsin-Madison and the University of Minnesota-Twin Cities. Talks and poster sessions will be presented by students, postdocs and faculty.
The 2013 IMV Symposium was held in May, 2013 and featured Vincent Racaniello as the Keynote Speaker as well as the recording of an episode of 'This Week in Virology'. Pictures from the IMV Symposium can be found on the IMV Facebook page and a videotape of the TWiV podcast can be viewed at www.twiv.tv
Read about bacteriophage phi 29 and why it matters.
Explore nearly a century's worth of discovery in the field of virology at the University of Minnesota.
"This Week in Virology" from professor Vincent Racaniello.