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Institute for Molecular Virology Home
Institute for Molecular Virology > Investigators > Core Investigators > Shelley Grimes

Shelley Grimes

Research Associate

Diag/Biological Sciences, School of Dentistry

Email: grime001@umn.edu
Phone: (612) 624-0667

Education

B.A., St. Olaf College
Ph.D., University of Minnesota
Postdoctoral Research: University of Minnesota

Research Interests

The 174-base bacteriophage phi29 prohead RNA (pRNA) is essential for invitro packaging of the 19-kilobase pair DNA-gp 3 complex (DNA-g3) into the viral precursor capsid (prohead). pRNA is an integral part of the phi29 DNA packaging motor, one of the strongest molecular motors characterized, pRNA forms a novel cyclic hexamer by intermolecular base pairing of identical molecules. This multimer binds to the head-tail connector of the prohead, the core of the packaging motor, where it appears as a pentameric ring by cryoEM 3-D reconstruction. A multimer of the packaging ATPase gp16 then binds the pRNA to complete the motor. pRNA is hypothesized to function in docking of the DNA-gp3 and the prohead, in recognition of the left end of DNA-gp3 to initiate packaging, and as a component of the DNA translocating ATPase. pRNA exits the DNA-filled head during neck and tail assembly, and it is not a part of the mature virion. Study of the structure and function
of this RNA-dependent DNA packaging motor may have general significance for assembly of other viruses, including mammalian viruses. The ultimate goal of this research is to determine the structures and the mechanisms by which pRNA constitutes the phi29 DNA packaging motor to catalyze DNA-gp3 translocation into the prohead.

Selected Recent Publications

  • Comolli, L.R., A.J. Spakowitz, C.E. Siegerist, P.J. Jardine, S.Grimes, D.L. Anderson, C. Bustamante and K.H. Downing. 2007. Three-dimensional architecture of the bacteriophage phi29 packaged genome and elucidation of its packaging process. Virology (in press).
  • Fuller, D.N., D. M. Raymer, J. P. Rickgauer, R. M. Robertson, C. E. Catalano, D. L. Anderson, S. Grimes and D. E. Smith. 2007. Measurements of Single DNA Molecule Packaging Dynamics in Bacteriophage lambda Reveal High Forces, High Motor Processivity, and Capsid Transformations. J Mol Biol. 373(5):1113-22. Epub 2007 Sep 11. PMID: 17919653.
  • Rickgauer, J.P., D. N. Fuller, S. Grimes, P.J. Jardine, D.L. Anderson, and D. E. Smith. 2007. Portal motor velocity and capsid pressure during viral DNA packaging in bacteriophage phi29. Biophys J. 2007 Sep 7; [Epub ahead of print]. PMID: 17827233.
  • Fuller, D., J. P. Rickgauer, P. J. Jardine, S. Grimes, D. L. Anderson and D. E. Smith. 2007. Ionic effects on viral DNA packaging and portal motor function in bacteriophage phi29. Proc. Natl. Acad. Sci. U. S. A; 104(27):11245-50.
  • Atz, R., S. Ma, J. Gao, D. L. Anderson, and S. Grimes. 2007. Alanine scanning and Fe-BABE probing of the bacteriophage phi29 prohead RNA-connector interaction. J. Mol. Biol. 369:239-248. PMID: 17433366.
  • Hugel, T., J. Michaelis,C. L. Hetherington, P. J. Jardine, S. Grimes, J. M. Walter, W. Falk, D. L. Anderson and C. Bustamante. Experimental Test of Connector Rotation during DNA Packaging into Bacteriophage varphi29 Capsids. PLoS Biol. 2007 Feb 20;5(3):e59 [Epub ahead of print].
  • Xiang, Y., M. C. Morais, A. J. Battisti, S. Grimes, P. J. Jardine,D. L. Anderson and M.G. Rossmann. 2006. Structural changes of bacteriophage phi29 upon DNA packaging and release. EMBO J. 25(21):5229-39.
  • Chemla, Y.R., K. Aathavan, J. Michaelis, S. Grimes, P. J. Jardine, D. L. Anderson and C. Bustamante. 2005. Mechanism of force generation of a viral DNA packaging motor. Cell. 122(5):683-92.
  • Wang, H., S. Grimes, D. L. Anderson, and P. Serwer. 2004. Terminal protein-induced stretching of bacteriophage phi29 DNA. J Microsc. 213:172-9.
  • Grimes, S., P. J. Jardine, and D. Anderson. 2002. Bacteriophage phi29 DNA packaging. Adv. Virus Res.58:255-294.
  • Smith, D. E., S. J. Tans, S. B. Smith, S. Grimes, D. L. Anderson and C. Bustamante. 2001. The bacteriophage straight phi29 portal motor can package DNA against a large internal force. Nature 413:748-52.
  • Morais, M. C., Y. Tao, N. H. Olson, S. Grimes, P. J. Jardine, D. L. Anderson, T. S. Baker, and M. G. Rossmann. 2001. Cryoelectron-microscopy image reconstruction of symmetry mismatches in bacteriophage phi29. J. Struct. Biol. 135:38-46.
  • Simpson, A. A., Y. Tao, P. G. Leiman, M. O. Badasso, Y. He, P. J. Jardine, N. H. Olson, M. C. Morais, S. Grimes, D. L. Anderson, T. S. Baker, and M. G. Rossmann. 2000. Structure of the bacteriophage phi29 DNA packaging motor. Nature. 408(6813):745-50.

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