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Perry Hackett

Professor

Genetics, Cell Biology and Development

Email: hacke004@umn.edu
Phone: (612) 624-6736

Education

Ph.D., University of Colorado, 1974

Research Interests

The Hackett lab's main area of interest is using transposons as vectors for gene therapy as well as tagging and mapping genes in vertebrate chromosomes. For this, we use the Sleeping Beauty Transposon System, so called because we awakened the transposons from an evolutionary sleep of more than 10 million years. The Sleeping Beauty system appears to be the most efficacious method for inserting precise, single expression cassettes into human chromosomes without using viruses. The lab works very closely with other labs in the Beckman Center for Transposon Research including the Ekker, McIvor and Largaespada labs. The lab is also developing transposon tools for studying gene expression in vertebrates and analyzing the roles of specific genes in vertebrate development.

Selected Recent Publications

• Hackett, C.S., A.M. Geurts and P.B. Hackett (2007) Predicting DNA vector insertion sites: Implications for functional genomics and gene therapy. Genome Biol. (in press)
• Aronovich, E.L., J.B. Bell, L.R. Belur, R. Gunther, B. Koniar, D.C. Erickson, P.A. Schachern, R.S. McIvor, C.B. Whitley and P.B. Hackett (2007). Sleeping Beauty transposon-mediated gene therapy of murine mucopolysaccharidosis. J. Gene Med. (in press). Online in advance of print: http://www3.interscience.wiley.com/cgi-bin/fulltext/114206158/HTMLSTART
• Hackett, P.B. (2007). Integrating DNA vectors for gene therapy. Mol. Ther. 15: 10-12.
• Balciunas, D., K.J. Wagensteen, A. C. Wilber, J.B. Bell, A.M. Geurts, S. Sivasubbu, X. Wang, P.B. Hackett, D.A. Largaespada, R.S. McIvor, and S.C. Ekker. (2006). Harnessing an efficient large cargo-capacity transposon for vertebrate gene transfer applications. PLoS Genetics.
• Wilber, A., J.L. Frandsen, A.M. Geurts, D.A. Largaespada, P.B. Hackett, and R.S. McIvor (2006). RNA as a source of transposase for Sleeping Beauty-mediated gene insertion and expression in somatic cells and tissues. Mol Ther. 13: 625-630.
• Geurts*, A.M., C.S. Hackett*, J.B. Bell, T.L. Bergemann, C.M. Carlson, L.S. Collier, D.A. Largaespada and P.B. Hackett (2006). Structure-based prediction of insertion-site preferences of transposons into chromatin. .Nucl. Acids Res. 34: 2803-2811.
• Score, P.R., L.R. Belur, J.L. Frandsen, J. Geurts, T. Yamaguchi, N.V. Somia, P.B. Hackett, D.A. Largaespada and R.S. McIvor (2006). Sleeping Beauty-mediated transposition and long-term expression in vivo: use of the LoxP-Cre recombinase system to distinguish transposition-specific expression. Mol. Therap. 13:617-624.
• Geurts, A.M., A.C. Wilber, C.M. Carlson, P.D. Lobitz, K.J. Clark, P.B. Hackett, R.S. McIvor, D.A. Largaespada (2006). Conditional gene expression in the mouse using a Sleeping Beauty gene-trap transposon. BMC Biotech 6(1): 30.85.
• Liu, G., A.M. Geurts, K. Yae, A.R. Srinivassan, S.C. Fahrenkrug, W.K. Olson J. Takeda, K. Horie and P.B. Hackett (2005). Target-site preference for Sleeping Beauty transposons. J. Mol. Biol. 346: 161-173.
• Ohlfest, J.R., J.L. Frandsen, S. Fritz, P.D. Lobitz, S.G. Perkinson, K.J. Clark, N.S. Key, R.. McIvor, P.B. Hackett and D.A. Largaespada (2005). Phenotypic correction and long-term Factor VIII expression in hemophilic mice by immunotolerization and nonviral gene transfer using the Sleeping Beauty transposon System. Blood 105: 2691-2698.